Bone regeneration effects of human allogenous bone substitutes: a preliminary study
이덕원, 구기태, 설양조, 이용무, 구영, 류인철, 정종평, 김태일,
소속 상세정보
이덕원 ( Lee Deok-Won ) - Seoul National University College of Medicine Department of Periodontology
구기태 ( Koo Ki-Tae ) - Seoul National University College of Medicine Department of Periodontology
설양조 ( Seol Yang-Jo ) - Seoul National University College of Medicine Department of Periodontology
이용무 ( Lee Yong-Moo ) - Seoul National University College of Medicine Department of Periodontology
구영 ( Ku Young ) - Seoul National University College of Medicine Department of Periodontology
류인철 ( Rhyu In-Chul ) - Seoul National University College of Medicine Department of Periodontology
정종평 ( Chung Chong-Pyoung ) - Seoul National University College of Medicine Department of Periodontology
김태일 ( Kim Tae-Il ) - Seoul National University College of Medicine Department of Periodontology
KMID : 0363020100400030132
Abstract
Purpose The purpose of this study was to compare the bone regeneration effects of cortical, cancellous, and cortico-cancellous human bone substitutes on calvarial defects of rabbits.
Methods Four 8-mm diameter calvarial defects were created in each of nine New Zealand white rabbits. Freeze-dried cortical bone, freeze-dried cortico-cancellous bone, and demineralized bone matrix with freeze-dried cancellous bone were inserted into the defects, while the non-grafted defect was regarded as the control. After 4, 8, and 12 weeks of healing, the experimental animals were euthanized for specimen preparation. Micro-computed tomography (micro-CT) was performed to calculate the percent bone volume. After histological evaluation, histomorphometric analysis was performed to quantify new bone formation.
Results In micro-CT evaluation, freeze-dried cortico-cancellous human bone showed the highest percent bone volume value among the experimental groups at week 4. At week 8 and week 12, freeze-dried cortical human bone showed the highest percent bone volume value among the experimental groups. In histologic evaluation, at week 4, freeze-dried cortico-cancellous human bone showed more prominent osteoid tissue than any other group. New bone formation was increased in all of the experimental groups at week 8 and 12. Histomorphometric data showed that freeze-dried cortico-cancellous human bone showed a significantly higher new bone formation percentile value than any other experimental group at week 4. At week 8, freeze-dried cortical human bone showed the highest value, of which a significant difference existed between freeze-dried cortical human bone and demineralized bone matrix with freeze-dried cancellous human bone. At week 12, there were no significant differences among the experimental groups.
Conclusions Freeze-dried cortico-cancellous human bone showed swift new bone formation at the 4-week healing phase, whereas there was less difference in new bone formation among the experimental groups in the following healing phases.
키워드
Bone Substitutes; Osteogenesis; Transplantation; X-Ray Microtomography
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